Image
|
Figure Caption
Fig. 29 Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014, with the exception that in situ hybridization was performed at 65oC. Primer sequences were: Forward: TGTGGCAAAAGATTGTCAGG. Reverse: AATTAACCCTCACTAAAGGGACGTGCAACAAAGAGGTTTGA. PCR product size: 1019 bp.
Figure Data