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Fig. 2

Cholinergic gene expression in zebrafish Hb-IPN; chatb (A) and vachtb (B) transcripts in the right dHb (brackets) and bilaterally in the vHb (arrowheads) of 4-dpf larvae (dorsal views). Double ISH for vachtb with chatb (C) and aoc1 (D). (E) hacta expression only in right dHb. (F) Confocal z-stack images of left and right dHb in Tg(slc17a6b:DsRed) larvae. Transverse sections through sst1-expressing IPN of 4-dpf larvae double-labeled for chrna2a (G), chrna2b (H), and chrnb4 (I). L, left; R, right. (Scale bars: 50 μm.)

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