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Fig. 2
Knockdown of hif2-alpha confers a small liver phenotype.
(A–J) Development of the embryonic liver was monitored by the expression pattern of the lfabp gene in wild-type embryos and hif2-alpha ATG-MO-injected embryos at 1–5 dpf by WISH, thereby revealing a small liver phenotype in hif2-alpha morphants. (K) Expression of the lfabp and fabp10a genes was investigated in wild-type embryos and hif2-alpha morphants at 5 dpf by qPCR (n = 30). Expression was normalized to β-actin. WT, wild type. The experiment was performed in triplicate, error bars indicate S.D. *, p<0.05, unpaired t-test.
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