|
Fig. 7
The effects of SU5402 and PD153035 on compound 10-induced angiogenesis in VRI-treated zebrafish.
(I) 24 hpf zebrafish embryos were treated with 0.1% DMSO (v/v) (A), 20 μM SU5402 (D) and 5 μM PD153035 (F) for 24 h. 21 hpf embryos were treated with VRI (500 ng/ml) for 3 h. After that, the VRI was washed out and replaced with 0.1% DMSO (v/v) embryo medium (B) or 0.3 μM 10 (C), 0.3 μM 10 and 20 μM SU5402 (E), 0.3 μM 10 and 5 μM PD153035 (G) for another 24 h. Yellow arrows indicated that the ISVs (intact and defective), SIVs, DLAVs, and DA. (II) Quantitative analysis of inhibitory effect of SU5402 and PD153035 on 10-induced angiogenesis in VRI-treated zebrafish. Data are expressed as mean±SEM, (ng3). **p<0.01 and ***p<0.001 versus the 10 treatment group.