|
Fig. 5
The effects of compound 10 on VRI-induced blood vessels loss in Tg(fli-1a:EGFP)y1 zebrafish compared to Tan IIA, CPT and Tan I.
(I) (A-A′) Control group: 24 hpf zebrafish embryos were treated with 0.1% DMSO (v/v) for 24 h and 48 h. 21 hpf embryos were treated with VRI (500 ng/ml) for 3 h. After that, the VRI was washed out and replaced with 0.1% DMSO (v/v) embryo medium (B-B′) or 10 [0.003 μM(C-C′), 0.01 μM(D-D′), 0.03 μM (E-E′), 0.1 µM (F-F′), 0.3 μM (G-G′),1 μM(H-H′)], 10 μM Tan IIA (I-I′), 3 μM CPT (J-J′) and 10 μM Tan I (K-K′)for 24 h and 48 h respectively. (a–k) Enlarged SIVs region (x 2.5) of (A′–K′) respectively. Yellow arrows indicated that the ISVs (intact and defective), SIVs, DLAVs, and DA. (II) Quantitative analysis of compounds on VRI-treated zebrafish. Number of defective and intact ISVs in each embryo was quantified by counting a minimum of 15 embryos per group at 48 hpt. Data are plotted as mean±SEM, (ng3). *p<0.05, **p<0.01 and ***p<0.001 versus the VRI-only treatment group.