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Fig. S4
Morphological analysis of CHX treated and pou5f1-VP16 mRNA injected control embryos. (A) Morphological phenotype of MZspg embryos treated with CHX from 64-cell stage on and developed until WT control embryos reached 60% epiboly. Treated embryos are arrested before sphere stage, but do not degenerate until 60% epiboly equivalent age. (B) The injection of 10 pg pou5f1-VP16 mRNA into 1-cell MZspg embryos is sufficient to rescue the MZspg phenotype, but it also may ventralize the embryo as Pou5f1 overexpression in WT would do [74]. The experiment demonstrates that pou5f1-VP16 was injected in our experiments at concentrations that could be considered physiological for embryonic development.