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Fig. 5

ID: ZDB-IMAGE-140812-3
Publication: Kotkamp et al., 2014 - Pou5f1/Oct4 promotes cell survival via direct activation of mych expression during zebrafish gastrulation
All Figures: Figures for Kotkamp et al., 2014

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Fig. 5

Analysis of the Mych contribution to the morphology of the MZspg mutant phenotype.

Knockdown of Mych function in WT by injecting splice-blocking (A-D) or translation-blocking (E-H) morpholinos. Morphological analysis of injected (A,C,E,G) and non-injected (B,D,F,H) embryos at 90%-epiboly and 25 hpf. Morphants show neither a delay in epiboly (A, E) nor obvious morphological defects by 25 hours development (C, G). Rescue of Mych activity in MZspg mutants by injecting mych mRNA into 1-cell stage embryos (I-L). Mych alone is not able to rescue the strong defects developed by Pou5f1 deficient embryos during gastrulation (I) and after 25 hpf (K).

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Phenotype details

Acknowledgments

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