ZFIN Image: Zaghloul et al., 2010, Fig. S1

Image

Figure Caption

Fig. S1

Suppression of bbs genes in vivo produces early gastrulation phenotypes. (A) Injection of splice-blocking (SB) MO against bbs1 or bbs6 results in complete suppression of message as shown by PCR amplification of cDNA reverse transcribed from extracted total mRNA. β-actin was used as a control. (B) SB MO produces similar phenotypes to those produced by translation-blocking MO and in similar proportions (C). Consistent with these similar defects, co-injection of SB MO with mutant mRNA for a subset of bbs1 or bbs6 mutations produced defects similar to those produced by coinjection with TB MO. (D-F) To verify specificity of each BBS mRNA to rescue the corresponding bbs MO, we attempted to rescue bbs1, bbs7, bbs4, bbs6, or bbs3 with either bbs7, bbs1, bbs6, or bbs4, respectively, and found that the mismatched mRNA did not rescue the morphant phenotypes.

Figure Data

Phenotype details

Acknowledgments

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