ZFIN Image: He et al., 2014, Fig. 1

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Figure Caption

Fig. 1

Detection of neuromast HCs in 5dpf larvae. (a) Confocal images of neuromasts from a 5dpf control larva and 5dpf larvae treated with 0.1μM TSA, 100μM VPA or 10μM MS-275. The number of neuromast HCs was assessed by GFP visualization in the transgenic line Tg(Brn3c:mGFP). There is a significant decrease in HC number in the larvae treated with HDAC inhibitors. HCs are green and nuclei stained with DAPI are blue. Scale bar=10μm. (b) The average number of HCs per neuromast (NM) in 5dpf zebrafish larvae. The number of GFP-positive cells decreased in larvae treated with HDAC inhibitors. The HCs of the first four neuromasts along the body, L1 to L4, were recorded on one side of each fish. Bars are mean�s.e.m. and n=total number of neuromasts. P<0.05. (c) The zebrafish larvae treated with 0.1μM TSA, 100μM VPA or 10μM MS-275 from 3dpf to 5dpf and control larvae were morphologically normal. (d) Superimposition of a fluorescent image of a 5dpf zebrafish larva with neuromast HCs labeled with GFP. White arrows point to primary neuromasts L1 to L8 on the left side of the larva and the image shows secondary neuromasts (*) and a few neuromasts from the right side of the larva (not labeled).

Acknowledgments

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