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Fig. S2 Expression pattern of march8 during embryogenesis. (A) Total RNA was extracted from staged embryos, and march8 mRNA was analyzed by RT-PCR; β-actin was used as a loading control. (B) March8 mRNA expression in zebrafish embryos was detected by in situ hybridization. Shown are 2 cell, 70% epiboly and tailbud stage; lateral views with animal pole on top. Three and 10 somite; left: lateral view with dorsal to the right; right: dorsal view anterior to the top. One and two day embryos; lateral view with anterior to the left.
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