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Fig. 4
C) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs. (B) Isl1 labels the pineal cells. (D–F) Knockdown of sox2 results in increased number of PhRs. (G) The average number of PhRs in control (purple bar) and sox2 morphants (orange bar). (H–J) Tg(elavl3:GFP) drives GFP expression specifically in the PNs. (K–M) The knockdown of sox2 does not affect the number of PNs. (N) The average number of PNs in controls (purple bar) and sox2 morphants (orange bar) does not significantly differ. Confocal maximum projections of 28 hpf embryos, scale bars = 25 μm, error bars represent ± standard error, ** = p-value <0.001 (MWU test). See also Figure S4.