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Fig. 3
Correlation among birefringence, acta1a:gfp, and phalloidin patterns.
Confocal images of a single control (A,C,E) and dmd-MO (B,D,F) larva at 4 dpf. Phalloidin staining was imaged using the red channel but false-colored in green in E,F. Lateral views of trunk somites show anterior to the left. The birefringence, acta1a-gfp, and phalloidin muscle lesion patterns strongly correlate in all larvae that were examined for all three patterns (n=8). Abnormal birefringence also correlates with lesions visualized with phalloidin staining in dmd-/- larvae (n=13, not shown).
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