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Fig. 3
vegfd is expressed in the head and pectoral fin buds. Representative images of whole-mount in situ hybridisation showing the expression of vegfc (A-E) and of vegfd (F-J), in 1.5dpf (A,F), 2dpf (B-D,G-I) and 3dpf embryos (E,J). (C,H) The dorsal aspect of the head region at 2dpf and (D,I) lateral aspect of the trunk region at 2dpf. vegfd expression in the pectoral fin buds is marked with black arrows in G,H,J. (A2,F2) Magnified images of the boxed regions in A and F; the black asterisks show the approximate position of the FLS. (K) qPCR analysis of vegfc and vegfd mRNA levels from isolated heads of 1.5, 3 and 5dpf embryos. Data are shown relative to mRNA levels in 1.5dpf embryos.