ZFIN Image: Swift et al., 2014, Fig. 4

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Figure Caption

Fig. 4 Sox gene function is required for nr2f2 expression in the zebrafish vasculature. (A and B) Whole mount in situ hybridization of 24 hpf control (A) and sox7/sox18 double morpholino (DMO) injected (B) zebrafish embryo trunks probed for nr2f2. The inset numbers in panels B show the number of in situ-stained embryos exhibiting strongly reduced or absent vascular expression over the total number of embryos examined. (C) Quantitative RT-PCR measurement of nr2f2 and lyve1 gene expression in excised trunks (see Materials and Methods of 24 hpf control and sox7/sox18 DMO animals. (D) Quantitative RT-PCR measurement of nr2f2 gene expression in excised trunks (see Materials and Methods) of 24 hpf control MO injected, sox7/sox18 DMO injected, cloche mutant, and sox7/sox18 DMO injected cloche mutant animals. All quantitative RT-PCR values are shown normalized to control gene expression levels, which are set equal to 1. Rostral is to the left and dorsal is up in all image panels. Scale bars, 50µm.

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Acknowledgments

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Reprinted from Developmental Biology, 390, Swift, M.R., Pham, V.N., Castranova, D., Bell, K., Poole, R.J., Weinstein, B.M., SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish, 116-25, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.