ZFIN Image: Bonetti et al., 2014, Fig. S2

Image

Figure Caption

Fig. S2 Heart looping defects in embryos expressing Shp2-T73I (NS) and Shp2- G465A (LS). Non-injected control embryos (NIC) and embryos injected at the one-cell stage with mRNA encoding WT-Shp2, Shp2-T73I (NS) or Shp2-G465A (LS) were fixed at 48 hpf and in situ hybridization was performed using probes for the myosin genes myl7 (A-D, cardiomyocytes), vmhc (E-H, ventricle), amhc (I-L, atrium), and for has2 (M-P, endocardial cushions). Representative pictures are shown and the number of embryos showing this pattern as well as the total number of embryos that were analyzed is indicated in the bottom right corner of each panel. The outline of the heart is indicated with a dashed line in panels M-P.

Acknowledgments

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