ZFIN Image: Weber et al., 2014, Fig. 1

Image

Figure Caption

Fig. 1

HC Precursors Divide at Multiple Positions but Do Not Account for All Nonapical Divisions

(A) Total number of pH3+ cells (gray), nonapically dividing cells (light blue), and Ptf1A+ nonapically dividing cells (dark blue) counted at each time point/embryo.

Error bars represent SD; n = 2–6 embryos per time point.

(B) Data from (A) as percentage of all counted mitoses. Error bars represent SD.

(C) Ptf1A:GFP (green) embryos stained for pH3 (red) and DAPI (blue). Ptf1A+/pH3+ cells are found adjacent to the OPL (top, arrows) and within the AC layer (bottom, arrows).

(D) Quantification of the location of Ptf1A+/pH3+ cells as percentage of pH3+ cells. Numbers on top of bars indicate number of dividing cells counted. n = 3 embryos/time point, pooled.

(E) INL division of a Ptf1A:GFP cell gives rise to two Ptf1A+ daughters (arrows). Time in hr:min. Imaging started at 46 hpf.

(F) pH3 staining (yellow) of a Ptf1a:dsRed/Cx55.5:rasGFP (magenta/green) embryo. All cells in the INL and adjacent to the OPL immunoreactive for pH3 express Ptf1A:dsRed and Cx55.5:rasGFP (arrows).

Scale bars represent 10 μm. See also Figure S1 and Movie S1.

Acknowledgments

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