ZFIN Image: Eroglu et al., 2014, Fig. S6

Image

Figure Caption

Fig. S6

HSBP1 is evolutionarily conserved and is expressed during early embryonic development in zebrafish.

(A) Human (hhsbp1), mouse (mhsbp1), rat (rhsbp1), Xenopus (xhsbp1), and zebrafish (zhsbp1) HSBP1 amino acids were aligned using CLUSTALW (http://pbil.ibcp.fr/htm/index.php). Conserved amino acids are highlighted in black.

(B) mRNA and cDNA were prepared from zebrafish embryos at different stages of development. Semiquantitative RT-PCR was performed using hsbp1 specific primers. β-Actin was used as a control. Negative control contained no input mRNA. (C) Whole mount in situ hybridization was performed to detect HSBP1 expression in wild-type zebrafish embryos collected at the 8-cell stage, and then at 3, 6, 10, 16 and 24 hpf. HSBP1 is strongly expressed maternally. During somitogenesis (16 hpf), tail shows reduced expression, and at 24 hpf the expression is limited to the head region. Mag. 40x.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 386(2), Eroglu, B., Min, J.N., Zhang, Y., Szurek, E., Moskophidis, D., Eroglu, A., and Mivechi, N.F., An essential role for heat shock transcription factor binding protein 1 (HSBP1) during early embryonic development, 448-460, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.