Fig. 3

Fig. 3 Homozyogus col8a1a mutant adults display vertebral fusions and scoliosis. MicroCT imaging of WT (A–B′) and col8a1a mutant zebrafish (C–D′) adults (45dpf) (A–E′′) in both lateral views (A, C, E–E′′) with insets (A′ and C′) and dorsal views (B and D) with insets (B′, D′) (A, A′, and B′) WT vertebrae with one neural and hemal arches per vertebral body. (B) Representative scoliosis in the tail of a WT adult (yellow arrowhead). (C and C′) Representative lev^vu41-/- zebrafish with VM (red arrowheads). (D and D′) Representative lev^vu41-/- multiple scoliosis along the axis (yellow arrowheads) and (E–E′′) Representative individuals scored for the presence of individual VM (red arrowheads) or complete fusion of vertebral column (red bracket) in the thoracic or caudal regions (as number of fused regions). (F) Graphed categorical data of VM from all lev alleles and unaffected WT siblings in thoracic (T) and caudal (C) regions. All scale bars (A–D), (A′–D′), and (E–E′′)=1 mM.