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Fig. 5 Expression domains delineate somitic primordia. (A) An injection in expression domain I (three brown cells at the arrow) in an embryo at the 3- somite stage. This embryo is one of the control cases, and was processed for in situ hybridization and anti-fluorescein antibody staining immediately after injection. (B) A fluorescence micrograph of an embryo in 5-somite stage that had been injected in domain I at the 3-somite stage. Labelling (arrow) is clearly visible within the 5th somite. (C) The same embryo after in situ hybridization and antifluorescein staining. I-III, expression domains. Scale bar, 100 μm.