ZFIN Image: Venkiteswaran et al., 2013, Fig. 2

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Figure Caption

Fig. 2

Cxcr7 Is Required for Sdf1a-GFP Sequestration by the Primordium

(A and B) Average fluorescence intensity of Sdf1a-GFP protein along the stripe of chemokine-producing cells underneath primordia in embryos of indicated genotypes (B, inset, heat-shocked wild-type control embryos).

(C) Sum projection of the primordium from 36 hpf embryos of indicated genotypes stained for Claudin-B and GFP. Scale bar, 10 μm. Direction of migration is to the right. Arrowheads indicate Sdf1a-GFP puncta.

(D) Distribution of intensities of Sdf1a-GFP puncta in primordia of indicated genotypes.

(E) Distribution of intensities of Sdf1a-GFP puncta in primordia along the anterior-posterior axis. Each dot (D and E) represents an individual punctum (red, cxcr7b^/; cxcr7a morphant primordia; black, wild-type primordia).

(F) Fraction of Sdf1a-GFP found inside of the primordium of the total Sdf1a-GFP in the indicated genotype. Each dot represents the fraction of Sdf1a-GFP in an individual primordium. Horizontal bars represent the mean ± SEM.

See also Figure S1 and Data S1 and S2.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Cell, 155(3), Venkiteswaran, G., Lewellis, S.W., Wang, J., Reynolds, E., Nicholson, C., and Knaut, H., Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue, 674-687, Copyright (2013) with permission from Elsevier. Full text @ Cell