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Fig. 2

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ZDB-IMAGE-140114-49
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Figures for Vo et al., 2013
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Fig. 2

Liver specific expression of fgb-egfp results in incorporation of fusion protein into an induced thrombus.

A fabp10-fgb-egfp transgenic line of zebrafish was produced, and F1 offspring analyzed. (a) Expression was liver specific, as demonstrated by the region of green fluorescence in a 5 dpf larva (yellow signal is yolk sac autofluorescence). (b, c) F1 adult fish displayed overt hemorrhage, suggestive of potential interference with coagulation secondary to dysfibrinogenemia. Pictures of the PCV prior to (d, brightfield+fluorescence; e, fluorescence only), and after (f, brightfield+fluorescence; g, fluorescence only) laser injury (f, g were prior to complete occlusion). The arrowhead in a indicates liver, arrows in d, f indicate PCV and direction of flow, and arrowheads in f, g point to the induced thrombus.

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