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Fig. 2

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ZDB-IMAGE-140107-41
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Figures for Reichert et al., 2013
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Fig. 2 cvl2 expression colocalises with domains of active BMP signalling and is required for NC induction. (A) ISH for bmp2b, mRFP and cvl2 in dorsal anterior view (top) and the same embryos turned 45° to the left (bottom). Arrows mark the sharp expression domain of mRFP and cvl2 at the NPB. (B) Timecourse of cvl2 expression. Germ ring to bud stage shown in lateral view, dorsal to the right. Somite stages are shown in dorsal anterior view, anterior to top. Arrows indicate the two domains of cvl2 expression. (C) DFISH reveals colocalisation of mRFP (red) and cvl2 (green) mRNA at the NPB. Dorsal anterior view. (D) DFISH for sox10 (red) and cvl2 (green). Lateral view with anterior to the right. Strong green staining reveals the outer domain of cvl2 expression (white arrow). Weaker green staining is visible in the NC (sox10; red arrow).

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