IMAGE

Fig. 6

ID
ZDB-IMAGE-131218-58
Source
Figures for Sarmah et al., 2013
Image
Figure Caption

Fig. 6 RA Supplementation during chronic ethanol treatment (2–48hpf) does not restore normal heart development. A: Schematic diagram showing the timing of ethanol and RA exposure. B–M: Live images of embryos at 28 (B–G) and 52 hpf (H–M) showed normal morphology in the control (B, H) and RA-treated (E, K) embryos. Ethanol-treated embryos exhibited small eye, shorter body (C, D, I, J) and pericardial edema (I, J). Ethanol+RA-treated embryos showed near normal eye size, slightly shorter body (F, G, L, M) and pericardial edema (L, M). Arrows, black, posterior end of the embryos; white arrow, eye, black arrowhead, pericardial edema. N–S: 3D renderings of fixed Tg[cmlc2:GFP] embryo confocal sections showed normal cardiac morphology in control (N) and RA-treated embryos (Q); straight hearts in ethanol-treated embryos (O, P); near normal or straight heart in ethanol+RA-cotreated (R, S) embryos. T–Y: Phalloidin-stained Tg[fli1:EGFP] embryos: clustering of GFP/F-actin-positive cells (white dashed line) at the AV boundary in the control (T) and RA-treated embryos (W); reduction and dispersion of GFP/ F-actin positive cells in ethanol-treated embryos (U, V); dispersion of GFP/F-actin-positive cells throughout the ventricle in ethanol+RA cotreated embryos (X, Y). A, anterior; P, posterior.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.