IMAGE

Fig. 2

ID
ZDB-IMAGE-131107-1
Source
Figures for Huang et al., 2013
Image
Figure Caption

Fig. 2

Laser Ablation of vSPNs Specifically Affects the First Undulation Cycle and Promotes Forward Swims during Phototaxis and the OMR

(A) Schematic of hindbrain spinal projection neurons (SPNs) of larval zebrafish (image modified from [11]).

(B) Two right MiV2 cells before and after laser ablation (arrows). The nearby ventral branch of the medial longitudinal fascicle (arrowhead) remains intact.

(C) Example of ablation phenotypes. Visually induced right turns are replaced by forward swims after ablation of the vSPNs on the right (right panels). The amplitude of the first tail bend (Θ1) is weaker, and the period (P1) of the first undulation is reduced. Turning to the nonlesion side is unaffected (left panels).

(D) Histograms of the change in heading direction (ΘΔH) before and after vSPN ablation. The unilateral ablation abolishes turning to the lesioned side (red arrow) while drastically increasing the occurrence of forward swims (red arrowhead). Data were collected from the same eight fish to plot the histograms in (D)–(K).

(E and F) Analysis of tail movements during phototaxis, represented by a 2D histogram with tail deflection (Θ) plotted on the x axis and cycle period (P) plotted on the y axis. The ablation affected the first undulation cycle (E), but not the later cycles (F). Dotted red line indicates the position of the preablation maximum.

(G) Histograms of the angle of the initial tail bend. The amplitude of bends toward the lesioned side is greatly reduced after the ablation. Instead, small-angle bends on either side of the body are performed (red arrowheads).

(H–K) During the OMR, the ablation also specifically affects the first undulation cycle of tail movements and promotes forward swims.

See also Figure S2.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.