ZFIN Image: Johnson et al., 2013, Fig. 2

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Figure Caption

Fig. 2

hoip embryos have myotube elongation defects. (A,B) Time-lapse images of rp298>τGFP embryos initiated at late St12. (A) Wild-type embryos showed robust myotube elongation at 30 minutes (double arrows) and developed extensive filopodia for attachment site recognition at 60 minutes (white arrows). (B) Myotubes established polarity in hoip¹ embryos at 15 minutes but failed to elongate by 30 minutes. Polarized myofibers at 15 minutes compacted over time (double-headed arrows). (C,D) St16 rp298>τGFP embryos double labeled for GFP and βPS. (C) βPS localizes to myotendinous junctions in wild-type embryos. (D) Tendon cells express βPS in hoip¹ embryos but localization is diffuse (red arrowheads). (C2,D2) βPS expression alone. (E,F) St16 rp298>τGFP embryos double labeled for GFP and Talin. Talin is expressed in tendon cells of wild-type (E) and hoip¹ (F) embryos. (E2,F2) Talin expression alone. mg, midgut. Scale bars: 20 μm.

Acknowledgments

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