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Fig. S8
nhp2l1b is a Hoip orthologue and the nhp2l1b ATG-MO efficiently blocks translation. (A) Alignment between Hoip and zebrafish nhp2l1b protein sequences. Identical amino acids are shaded black; similar amino acids are shaded gray. (B) Alignment of the nhp2l1a/b translational start sequences with the ATG-MO and 5′UTR-MO. Target nucleotides are red. The beginning of the ORF is underlined. (C) Phylogenetic analysis of NHP2 and NHP2L1 proteins in worms (ce), flies (dm), zebrafish (dr) and humans (hs). Bootstrap values are given. (D) Zebrafish embryos co-injected with Control-MO and an eGFP construct containing the nhp2l1b target site in the 52UTR show robust eGFP expression at 12 hpf. (E) Embryos co-injected with ATG-MO and the same eGFP construct as in D show a significant reduction in eGFP expression. (F,G) Tg(α-actin:GFP) embryos injected with ATG-MO did not develop beyond 16 hpf.