ZFIN Image: Johnson et al., 2013, Fig. S3

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Figure Caption

Fig. S3

Muscle morphology and identity gene expression. (A, B) Nau expression in St12 embryos. The number of Nau+ nuclei is comparable between control and hoip¹ embryos. (C, D) rp298.lacZ expression in St12 embryos. The number of lacZ⁺ nuclei is comparable between control and hoip¹ embryos. (E, F) St16 rp298.gal4>τ.GFP, rp298.nlacZ embryos double-labeled for GFP (red) and lacZ (green). hoip¹ embryos showed a significant reduction in the number of lacZ⁺ nuclei (F) compared with wild type (E). (G, H) Quantification of Nau⁺ and rp298.lacZ⁺ positive nuclei in the dorsal mesoderm. The number of segments quantified is given for each genotype and time point. Unpaired t-tests were performed to establish significance. Error bars represent s.e.m. (I, J) MHC.τGFP, Hand. nGFP expression in St16 embryos. (I) Wild-type and (J) MHC¹ embryos show similar somatic muscle morphology. In particular LL1/DO5 muscles have elongated and attached in all segments (red arrows). ns, not significant; ***P<0.001.

Acknowledgments

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