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Fig. S1 WNT and FGF signaling do not regulate expression of cited3. We utilized the hsp70: Δtcf-gfp transgenic line to block Wnt signaling by expression of dominant-negative tcf (A,B), the hsp70:wnt8-gfp transgenic line to overactivate Wnt signaling by expression of wnt8 (C,D) and the hsp70:dnfgfr1 transgenic line to block Fgf signaling by expression of dominant negative fgfr1 (E,F). Embryos were collected by mating heterozygous transgenic animals. At 15 hpf, embryos were heatshocked for 1 hour at 37°C and were revived for 2 h at 28°C. As expected 25% of the embryos (wild-type control siblings) did not express the transgene (A,C,E), while the remaining 75% of the embryos expressed the respective transgene (B,D,F). Expression of cited3 was not affected when WNT signaling was blocked (A,B), or over activated (C,D). Expression of cited3 was also not disturbed when FGF signaling was blocked (E,F) at 18 hpf.