Fig. 5 Other Members of the Kalihinol Family also Show Copper Chelating Activity (A) Chemical structure of kalihinol analogs. (B) Seven hr postfertilization embryos were treated with either 25 μg/ml or 2.5 μg/ml of kalihinol analogs and DMSO vehicle control (first, second, and third columns). For phenotype rescue experiments, 5 hpf embryos were treated with kalihinols and given CuCl2 at 7 hpf (fourth, fifth, and sixth columns). Embryos were monitored for wavy notochord (black arrow), loss of pigmentation (blue arrow), and hematopoiesis (red arrow).(C) Effects of addition of Cu2+ to the 1H NMR line widths of the methyl signal region of pulcherrimol.
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