ZFIN Image: Sacilotto et al., 2013, Fig. 4

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Figure Caption

Fig. 4

Combinatorial knockdown of sox7, sox18, and rbpj in zebrafish results in ablation of both Dll4in3:GFP and endogenous dll4 expression. (A) Analysis of GFP expression in scrambled, rbpj, double sox7;sox18, and triple sox7;sox18;rbpj MO-injected tg(Dll4in3:GFP) embryos at 34 hpf. (B) Analysis of endogenous dll4 gene expression in scrambled, rbpj, double sox7;sox18, and triple sox7;sox18;rbpj MO-injected WT zebrafish embryos at 26 hpf. dll4 expression was detected by in situ hybridization. Values on the bottom right indicate number of embryos with the predominant and displayed phenotype per total number of embryos analyzed. In all cases, 0.125 pmol of sox7 and sox18 and 0.15 pmol of rbpj MO were used. *, highlights ectopic neural expression; arrowhead shows dorsal aorta. See also Fig. S6.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA