ZFIN Image: Clancey et al., 2013, Fig. 8

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Figure Caption

Fig. 8

ALLM treatment reduces accumulation of 62 kDa PARP-1 fragment.

A) Immunoblot analysis of PARP-1 cleavage fragments in wildtype (AB) and vps11^w66 larvae lysates. Treatment of larvae with 10 μM ALLM reduces the 62 kDa fragment (indicative of cathepsin activity; indicated by a bracket) below untreated levels. We also detect a 40 kDa cleavage product, which potentially represents calpain activity (see results text for additional details). B) Graphical representation of density analysis of 62 kDa band from three immunoblots using Image J. Samples were analyzed via one way ANOVA with Bonferonni multiple comparison analysis (p<0.05*, p<0.001**). Error bars are standard deviation.

Acknowledgments

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