ZFIN Image: Villefranc et al., 2013, Fig. S1

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Figure Caption

Fig. S1 Transgenic haploid screen in Tg(fli1a:egfp)^y1 zebrafish to identify mutants affecting Vegfc/Flt4 signaling. (A) Tg(fli1a:egfp)^y1 males were treated with N-ethyl-N-nitrosourea (ENU) to induce mutations in the pre-meiotic germline and subsequently outcrossed to wild-type Tg(fli1a:egfp)^y1 females to establish F1 families. Eggs from individual F1 females were fertilized in vitro with UV-irradiated sperm from wild-type males to generate haploid embryos. (B) Epifluorescence images of cranial blood vessels in Tg(fli1a:egfp)^y1 haploid embryos. (Left) Normal primordial hindbrain channel (PHBC) formation (arrows) at 28 hpf. (Right) Putative mutant embryo (‘putant’), which failed to form a PHBC (arrowheads denote location where PHBC normally forms).

Acknowledgments

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