A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development
- Authors
- Villefranc, J.A., Nicoli, S., Bentley, K., Jeltsch, M., Zarkada, G., Moore, J.C., Gerhardt, H., Alitalo, K., and Lawson, N.D.
- ID
- ZDB-PUB-130322-28
- Date
- 2013
- Source
- Development (Cambridge, England) 140(7): 1497-1506 (Journal)
- Registered Authors
- Lawson, Nathan, Moore, John, Nicoli, Stefania, Villefranc, Jacque
- Keywords
- Vegfc, angiogenesis, lymphatic, zebrafish
- MeSH Terms
-
- Paracrine Communication/genetics
- Paracrine Communication/physiology
- Blood Vessels/embryology*
- Blood Vessels/growth & development
- Zebrafish/embryology
- Zebrafish/genetics
- Vascular Endothelial Growth Factor C/genetics*
- Vascular Endothelial Growth Factor C/physiology*
- Protein Isoforms/genetics
- Protein Isoforms/physiology
- Signal Transduction/genetics
- Cell Movement/genetics
- Cell Movement/physiology
- Mice
- Embryo, Nonmammalian
- Mice, Knockout
- Autocrine Communication/genetics
- Autocrine Communication/physiology
- Codon, Nonsense/physiology
- Alleles
- Neovascularization, Physiologic/genetics
- Neovascularization, Physiologic/physiology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- Lymphatic System/embryology*
- Lymphatic System/growth & development
- Female
- Animals, Genetically Modified
- Animals
- PubMed
- 23462469 Full text @ Development
Vascular endothelial growth factor C (Vegfc) is a secreted protein that guides lymphatic development in vertebrate embryos. However, its role during developmental angiogenesis is not well characterized. Here, we identify a mutation in zebrafish vegfc that severely affects lymphatic development and leads to angiogenesis defects on sensitized genetic backgrounds. The um18 mutation prematurely truncated Vegfc, blocking its secretion and paracrine activity but not its ability to activate its receptor Flt4. When expressed in endothelial cells, vegfcum18 could not rescue lymphatic defects in mutant embryos, but induced ectopic blood vessel branching. Furthermore, vegfc-deficient endothelial cells did not efficiently contribute to tip cell positions in developing sprouts. Computational modeling together with assessment of endothelial cell dynamics by time-lapse analysis suggested that an autocrine Vegfc/Flt4 loop plays an important role in migratory persistence and filopodia stability during sprouting. Our results suggest that Vegfc acts in two distinct modes during development: as a paracrine factor secreted from arteries to guide closely associated lymphatic vasculature and as an autocrine factor to drive migratory persistence during angiogenesis.