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Fig. 5 The interaction between interrenal and chromaffin cells in the fn1 mutant at 77 hpf. Double ISH assays showing the colocalization of ff1b (red) with dbh (black) at 77 hpf in the class I and II fn1 mutants, as well as their wild-type sibling. Ventral flat mount (A–A′′, C–C′′, E–E′′) and lateral (B–B′′, D–D′′, F–F′′, G–G″) views are shown for the representative embryo of each phenotypic type (n = 14, 7, and 58 for the fn1 mutant class I, class II, and their wild-type siblings respectively). The anterior (A) versus posterior (P) orientation of each sample is indicated. The embryos in the lateral view panels are oriented with the dorsal side to the top. Defective migration and convergence of chromaffin cells in the fn1 mutant result in incomplete interrenal organ assembly. Scale bar = 50 μM.