ZFIN Image: Tao et al., 2013, Fig. 3

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Figure Caption

Fig. 3 The def transgene in Tg-I rescues the liver but not intestine and exocrine pancreas development in def^hi429.

(A, B) WISH was performed to analyze the digestive organs in embryos at 4dpf produced by Tg-I^+/ def^hi429/+ × def^hi429/+ using the liver marker fabp10a, intestinal marker fabp2 and exocrine pancreas marker trypsin (A). Embryos from (A) were then individually genotyped and matched to their phenotype. Number of embryos identified in each category was as shown in (B). (C) Progenies from Tg-I^+/ def^hi429/+ × def^hi429/+ at 3.5dpf were also examined simultaneously by Fast Red-labeled fabp10a and trypsin probes BCIP/NBT-labeled fabp2 and insulin probes. Number of embryos in each category was obtained as shown after genotyping a total of 51 embryos from the cross. en: endocrine pancreas, ex: exocrine pancreas, in: intestine, lv: liver.

Figure Data

Phenotype details

Acknowledgments

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