Fig. 6 Gentamicin treatment resulted in fewer saccular hair bundles after 24 hours.
Adult wildtype zebrafish received a single 250 mg/kg intraperitoneal injection of gentamicin and allowed to recover for 24 hours. Images of phalloidin-labeled cells were taken at (A, F) 5%, (B, G) 25%, (C, H) 50%, (D, I) 75%, and (E, J) 95% along the length of the saccule beginning at the rostral end in (A–E) untreated controls and (F–J) gentamicin-treated animals. (K) Mean (± SD) number of saccular hair cell bundles per 900 μm2 of epithelia. Significantly fewer phalloidin-labeled hair bundles were counted at each area along the length of the saccule in gentamicin-treated animals compared to controls. *p<0.05 or **p<0.01; n = 6–13 saccules per area per condition.