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Fig. 3
HOXC9 overexpression rescues the defects in parachordal lymphangioplast (PL) formation in Stab2 morphants.
(A) Overall morphology of 48 hpf zebrafish embryo after control morpholino injection. Red box shows region displayed in (B–D). (B) Normal formation of the PLs (arrows) in 48 hpf tg(fli1:EGFP) zebrafish embryo after injection of 4 ng control morpholino. (C) Silencing of Stab2 expression using 4 ng splice-blocking morpholino disrupted the formation of the PLs (asterisks) in 48 hpf tg(fli1:EGFP) zebrafish embryo. (D) Injection of HOXC9 mRNA (50 pg) rescued the Stab2 loss-of-function phenotype in 48 hpf tg(fli1:EGFP) zebrafish embryo. (E) Quantification of 48 hpf tg(fli1:EGFP) fish embryos showing a disturbed PL formation including rescue experiments using HOXC9 mRNA (50 pg). Embryos were divided in three groups depending on the PL appearance being completely absent, partially formed or completely present. (F) Functionality of the mRNA injection. Western Blot of Orange-mRNA (50 pg) and HOXC9-mRNA (50 pg) injected zebrafish embryos at 24 hpf. (G) Quantification of (F), n = 3 per group. *p<0.05 vs. Orange-mRNA. Black scale bar: 500 μm. White scale bar: 50 μm.