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Fig. S12
Actinodin alignments across vertebrates. a) A MultiPipMaker global alignment of zebrafish and1 (actinodin) syntenic regions (around 40k bases) among three fishes, zebrafish, Medaka and stickleback, and one amphibian Xenopus tropicalis. The comparison was made against the zebrafish sequence. All sequences were extracted from Ensembl genomic databases available at the Welcome Trust Sanger Institute, Genome Research Limited. In Xenopus, the comparable conserved elements to fish and1 were not observed in the sequence between adipoq and myeov2. adipoq: adiponectin; myeov2: myeloma overexpressed2; otos: otospiralin. The annotation of the zebrafish and1 was made using NCBI GenBank accession NM_00119725 and Zhang et al. (2010). MultiPipMaker: http://pipmaker.bx.psu.edu/pipmaker/ (Schwartz et al., 2000). Note that the teleost fish Atlantic cod (Gadus morhua) does have adipoq, and1, myeov2, and otos but their syntenic relationships are not yet determined (Ensembl genomic databases at the Welcome Trust Sanger Institute, Genome Research Limited, updated on January 17, 2012). b) A MultiPipMaker alignment of zebrafish and Latimeria chalumnae and1 (actinodin) syntenic regions among different vertebrate animals. The comparison was made against the zebrafish. The conserved and1 element was only found in medaka, stickleback and L. chalumnae when the zebrafish and1 syntenic region was compared against the regions of the other vertebrates. adipoq: adiponectin; myeov2: myeloma overexpressed2; otos: otospiralin.