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Fig. S2 Overall strategy for co-expression of oncogenes in zebrafish. Stable transgenic lines expressing zebrafish Smoa1 were generated using a Tol2-based vector (A). Constitutively active human AKT1 (myrhAKT1) was incorporated into a meganuclease-based vector (B). The zebrafish krt4 promoter could simultaneously activate smoa1-EGFP and myrhAKT1 expression through Gal4VP16-UAS.
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