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Fig. 1

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ZDB-IMAGE-130306-1
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Figures for So et al., 2013
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Fig. 1 Non-hepatic endodermal cells are directly converted into hepatoblasts upon Wnt8a overexpression.

(A,B) Wild-type or Tg(hs:wnt8a) embryos were heat-shocked at 26hpf, harvested at 40hpf, and processed for immunostaining with anti-Prox1 (green) and anti-Pdx1 (red) antibodies. Ectopic hepatoblasts were present in the endoderm posterior to the liver-forming region in Wnt8a-overexpressing embryos (B, bracket). (C,D) anti-Prox1 and anti-Pdx1 staining of wild-type embryos at 26hpf and a schematic representation. Prox1 is expressed in endodermal cells at the 1st to 3rd somite levels, but not at the 4th to 6th somite levels. S1–S6 denotes the 1st–6th somite; the pink region indicates the dorsal pancreas (DP). Dashed lines in D outline the Pdx1+ region beneath the liver. (E) Cartoons illustrating cell-lineage tracing experiments. (F,G) Wild-type or Tg(hs:wnt8a) embryos were co-injected with sox32 and kikume mRNA into a single cell at the 32-cell stage, resulting in the mosaic expression of Kikume in the endoderm. Endodermal cells at the 5th somite level were photoconverted at 25.5hpf and then heat-shocked. Excited cells emit red fluorescence (KikRed) instead of green (KikGreen). The embryos were harvested at 40hpf and processed for immunostaining. To reveal the entire endoderm morphology including the dorsal pancreas (dotted lines), both Pdx1 and Kikgreen expression, which was detected using the same laser, are shown. KikRed+ cells expressed Prox1 (green) in Wnt8a-overexpressing embryos (G, open arrowheads), but not in controls (F, arrowheads). (H–H3) Higher magnification images of the square region in G. (I) Cartoon incorporating all lineage-tracing data. Green and blue lines outline Prox1+ and Pdx1+ domains, respectively, and n indicates the number of embryos. Dotted lines outline the dorsal pancreas (DP). All confocal images are ventral views with anterior up. Scale bars, 20µm.

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