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Fig. 6 Necrotic myocyte cell death induced by H/R in vivo.
Necrotic myocyte cell death was assessed under baseline conditions, and 18 h and 30d after H/R in the Tg(cmlc2:nucDsRed) zebrafish line. At 18 h after H/R it was found a marked increase in necrotic myocardial cell number, which was back to control value at the 30d time point. (a) Representative image of a zebrafish heart ventricular section 18 h after H/R showing colocalization of DAPI, DsRED and AO stainings. Arrows indicate cardiomyocyte AO+ nuclei. (b) AO+ cardiomyocytes nuclei in control (C) animals, and 18 h and 30d after H/R (n = 3 at each time point; ** p<0.01 vs. C).