IMAGE

Fig. 4

ID
ZDB-IMAGE-130117-45
Source
Figures for Chu et al., 2012
Image
Figure Caption

Fig. 4 Rock1, but not Rock2a, is necessary for YSL formation in the zebrafish embryo.>

(A) Protein domains of Rock1 and Rock2a and the putative dominant-negative (dn) construct. (B) Schematic of single-cell injection into the 2-cell stage embryo. (C) Embryos at oblong stage were stained for β-tubulin (white), F-actin (red) and DNA (blue). Images are projections of multiple focal planes. Arrowheads in Ci′,i′′ indicate a mitotic cell in late anaphase. Injection of dn-Rock2a (Cii–ii′′) did not affect YSL formation. Injection of dn-Rock1 (Ciii–iii′′) caused expansion of the YSL. (D) Statistical data for embryos expressing dn-Rock1 showing dose-dependent YSL expansion.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open