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Fig. 4 DFC clustering is disturbed in lpar3 and atx morphants during epiboly. (A-C) Zebrafish embryos injected with the indicated MOs were subjected to WISH against sox17 at 6 hpf. In contrast to the ovoid-shaped DFCs cluster in Std MO-injected embryos (A), dispersed DFC clusters were observed in both lpar3 (B) and atx (C) morphants. (D-I) Tg (sox17:GFP) embryos were MO injected, cultured to 7 hpf and 8 hpf, and photographed under epifluorescence DIC microscopy. The DIC and epifluorescence images are superimposed and presented. More spread-out and linear-shaped DFCs were observed in both lpar3 (E,H) and atx (F,I) morphants compared with Std MO-injected embryos (D,G).