Fig. 2 Comparison of unc45a and unc45b mRNA expression at 48 hpf.
Wild type siblings (a,e,e′); unc45a-/- (b,f,f′); unc45b-/- (c,g,g′); and unc45b-/-; unc45a-/- (d,h,h′) mutants. unc45a expression is diffuse in the region of the brain and pharyngeal arches. unc45b mRNA expression from lateral (e–h) and dorsal (e′–h′) views, head to the left. Wild type siblings (e,e′) and unc45a-/- (f,f′) mutants express unc45b in the extraocular (Eo), cardiac (Ca), trunk (Tr), and pectoral fin (Pf) muscles. Expression is either minimal or absent in the extraocular, cardiac, and pectoral fin muscles of the unc45b-/- (g,g′) and unc45b-/-; unc45a-/- (h,h′) mutants while expression in the trunk musculature appears to up-regulated in these mutants. Apostrophes denote alternative views of the same embryo.