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Fig. 1 Non-muscle myosin is required for apical constriction-driven proneuromast assembly. (A-D′′) Live images of the primordium of a 32 hpf zebrafish embryo co-expressing gfp-myh9 fusion (green) and lynTdTomato (red) mRNAs. B-D′′ are close-up views of the three boxed areas in A. (E-G′) Side view of the primordium of a 32 hpf cldnb:gfp embryo immunostained with Phospho-Myosin Light Chain 2 (ser19) (PMLC2) and GFP antibodies. F-G′ are close-up views of the two boxed areas in G. (H,I) Live images of the primordia of 32 hpf embryos treated with DMSO (H) or with 50 μM blebbistatin. Scale bars: 20 μm in A; 10 μm in B-D′′.