PUBLICATION

Shroom3 is required downstream of FGF signalling to mediate proneuromast assembly in zebrafish

Authors
Ernst, S., Liu, K., Agarwala, S., Moratscheck, N., Avci, M.E., Nogare, D.D., Chitnis, A.B., Ronneberger, O., and Lecaudey, V.
ID
ZDB-PUB-121127-2
Date
2012
Source
Development (Cambridge, England)   139(24): 4571-4581 (Journal)
Registered Authors
Chitnis, Ajay, Lecaudey, Virginie
Keywords
lateral line, apical constriction, rosette, shroom3, rho kinase, collective cell migration, morphogenesis, zebrafish
MeSH Terms
  • Microfilament Proteins/genetics
  • Microfilament Proteins/metabolism
  • Microfilament Proteins/physiology*
  • Cell Polarity/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Myosins/metabolism
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • Embryo, Nonmammalian
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Lateral Line System/embryology*
  • Lateral Line System/metabolism
  • Lateral Line System/physiology
  • Tissue Distribution/genetics
  • Animals, Genetically Modified
  • Cell Movement/genetics
  • Cell Movement/physiology
  • Mechanoreceptors/cytology
  • Mechanoreceptors/metabolism
  • Mechanoreceptors/physiology*
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Fibroblast Growth Factors/metabolism*
  • Fibroblast Growth Factors/physiology
  • Morphogenesis/genetics*
  • Morphogenesis/physiology
(all 30)
PubMed
23136387 Full text @ Development
Abstract

During development, morphogenetic processes require a precise coordination of cell differentiation, cell shape changes and, often, cell migration. Yet, how pattern information is used to orchestrate these different processes is still unclear. During lateral line (LL) morphogenesis, a group of cells simultaneously migrate and assemble radially organized cell clusters, termed rosettes, that prefigure LL sensory organs. This process is controlled by Fibroblast growth factor (FGF) signalling, which induces cell fate changes, cell migration and cell shape changes. However, the exact molecular mechanisms induced by FGF activation that mediate these changes on a cellular level are not known. Here, we focus on the mechanisms by which FGFs control apical constriction and rosette assembly. We show that apical constriction in the LL primordium requires the activity of non-muscle myosin. We demonstrate further that shroom3, a well-known regulator of non-muscle myosin activity, is expressed in the LL primordium and that its expression requires FGF signalling. Using gain- and loss-of-function experiments, we demonstrate that Shroom3 is the main organizer of cell shape changes during rosette assembly, probably by coordinating Rho kinase recruitment and non-muscle myosin activation. In order to quantify morphogenesis in the LL primordium in an unbiased manner, we developed a unique trainable ?rosette detector?. We thus propose a model in which Shroom3 drives rosette assembly in the LL downstream of FGF in a Rho kinase- and non-muscle myosin-dependent manner. In conclusion, we uncovered the first mechanistic link between patterning and morphogenesis during LL sensory organ formation.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m1274TgTransgenic Insertion
    m1275TgTransgenic Insertion
      pd1TgTransgenic Insertion
        zf106TgTransgenic Insertion
          zf115TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            shroom3MO1-shroom3MRPHLNO
            1 - 1 of 1
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            Fish
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            TagRFPEFGTagRFP
            1 - 3 of 3
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            Mapping
            No data available