Fig. 3 Effects of transgene activation on expression of the Fgf reporter etv5b.
All embryos were heat shocked for 30 minutes beginning at 24 hpf. Wild-type and hs:fgf8 embryos were heat shocked at 39°C and hs:dnfgfr1 embryos were heat shocked at 38°C. (A-C) Cross sections showing etv5b expression in wild-type (A), hs:fgf (B) and hs:dnfgfr1 (C) embryos at 26 hpf. (D-I) Dorsal views (anterior up) of wholemounts showing etv5b expression in wild-type (D, G), hs:fgf8 (E, H) and hs:dnfgfr1 (F, I) embryos at the indicated times. The otic vesicle (ov) is marked. Expression of etv5b remains elevated in hs:fgf8 embryos for at least 6 hours after heat shock, whereas etv5b expression is downregulated in hs:dnfgfr1 for at least 12 hours after heat shock. Scale bar, 25 μm.