ZFIN Image: Mackenzie et al., 2012, Fig. 5

Image

Figure Caption

Fig. 5 Flubendazole impairs division of hair cell progenitors.

ET4:GFP larvae were treated at 5 dpf with neomycin or copper for 1 h and incubated in flubendazole for 24 h. Immunohistochemistry was performed for GFP and PHH3, which is upregulated during mitosis. (A) Two-way ANOVA followed by Bonferroni post-hoc analysis found significant increases in the proportion of PHH3-positive cells in neomycin- and copper-treated larvae when treated with flubendazole (*, p<0.05; ***, p<0.001), demonstrating that cell division was arrested in GFP-positive hair cell precursors. N = 8 fish per group. Error bars are + SD. (B) Example neuromast at 24 hpt from a fish treated with neomycin and flubendazole. Arrows mark PHH3-positive nuclei.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One