Fig. 5

Fig. 5 Phenotype of liver-specific supv3l1 inactivation. (A–D) Confocal images of the Left (A and B) and Right (C and D) sides of 21-d-old LiPan (A and C) and LiPan/supv3l1^LKO (B and D) fish showing detached hepatocytes (white arrows) (B and D) and smaller right liver lobe (white arrow) in supv3l1^LKO fish (D). (E and F) Histological analysis of wild-type (E) and supv3l1^LKO (F) fish indicate disorganized liver with dead cells (arrowhead) but normal intestinal lumen (black arrows) in supv3l1^LKO fish (F). (F) Survival curve of supv3l1^LKO fish (lens YFP⁺) compared with supv3l1^GtNf/GtNf siblings (lens YFP^-) during the first 6 wk of life. One group was fed a larval diet until 28 dpf followed by a juvenile diet (red) and the other group was kept on the larval diet (blue). (G) Photograph of a wild-type and a supv3l1^LKO mutant at 5 wk of age showing the smaller size of the mutant.