ZFIN Image: Kizil et al., 2012, Fig. S5

Image

Figure Caption

Fig. S5 cxcr5 translation-blocking antisense morpholino is functional. (A) Uninjected 2 day post-fertilization (dpf) embryos. (B) Control morpholino-injected embryos show no morphological phenotypes at 2 dpf. (C)cxcr5 antisense morpholinoinjected embryos display severe anomalies in axial extension and head development. (D)cxcr5 mRNA rescues the knockdown phenotypes when co-injected with antisense morpholinos. (E) Quantitative real-time PCR analysis of genes regulated by cxcr5, upon control and antisense morpholino injections. All the genes tested are upregulated after knocking down cxcr5. Percentages in A-D represent the ratio of embryos with gross morphological defects to the whole clutch size.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.